Saccamoeba limax (Dujardin, 1841) Page, 1974

Saccamoeba limax 1-6: locomotive; 7,11: cell when changing the direction of movement; 8: resting cell; 9-10, 12: villous-bulbous uroid 13: nucleus (stained preparation).
Scale bars 1-11: 20 mkm; 12-13: 10 mkm

Saccamoeba limax 1: nucleus (Glu-Os fixation); 2: nucleus (Os fixation); 3: nucleus (Os-Glu-Os fixation); 4-5: filamentous inner nuclear lamina; 6: cell coat (Os fixation); 7: cell coat (Glu-Os fixation); 8-9: mitocahondria; 10: dyctiosome; 12: bacteria in the cytoplasm.
Scale bars 1-5, 8-12: 500nm; 6-7: 250 nm

  • Locomotive form monopodial, always without hyaline cap. Villous-bulbous uroid in most cells. Length in locomotion 49-80 mkm (average 63 mkm), breadth 10-18 mkm (average 13 mkm). L/B about 5.0.
  • Single spherical nucleus, about 6 mkm in diameter, with central endosome about 3 mkm in diameter. One contractile vacuole. 10-30 large bipiramidal or plate-like cristals in the cytoplasm.No cysts found.
  • Fine structural pattern depends greatly on the fixation procedure. Best results were observed with Os fixation.
  • Spherical nucleus with loose nucleolus and (in some) several peripheral nucleoli. Fibrous inner nuclear lamina. Cell coat in best fixed specimens consists of tightly packed prismatical elements, about 15 nm in thickness. In ordinar fixations it looks amorphous, with the evidence of cup-like structures. Mitochondria have long, sometimes helical cristae. Dictyosomes are not numerous, consist of 4-6 cisternae. In most cells there are bacteria, lying freely in the cytoplasm.

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