2. Sampling sites
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3. Study of amoebae fauna
4. Differential counting of amoebae
An original counting method, illustrated below was elaborated and applied
in course of this work since 1992,
however actually was published only in 1998 (delay of the journal appearance)
(Smirnov et al., 1998. Proc. St. Petersburg nat. Soc. s.1, v.
92: 74-81. ). This method was found to be much more suitable for field work than
routine MPN methods. Verification of this method, done by mean of
repetitive countings from the same well-mixed, homogenous sample indicated
the deviation 7-18%, which is much lower that the deviation on the order of
magnitude, shown by MPN methods (method by Singh (1946: Ann. Appl. Biol. 33: 112-119) was used).
Very similar approach was suggested by Anderson & Rogerson (1995: Europ.
J.Protistol. 31: 223-233)
Method of amoebae counting, used in this work (above ref: Smirnov, 1998)