Amoeba borokensis CCAP 1503/7.
Inverted NikonDiaphot, phase contrast.
Locomotive forms, nucleus and uroidal structures.
Amoeba borokensis Kalinina, Afon'kin, Gromov, Khrebtukova & Page, 1987
Lentgh in locomotion 210-545 mkm (average about 370 mkm).
Majority of amoebae are polypodial (polytactic) under favourable
conditions, with small minority orthotactic.
Floating form of radial type; several thick pseudopodia of unequal
lenght radiate from irregular central mass of the granuloplasm.
Most of crystals rectangular plates.
Nucleus of granular type, a compress sphere or ovoid,
discoid, but not concave, 23-37 mkm in diameter (average 29 mkm).
Surface coat consists of crinkly filaments
200-250 nm above the plasma membrane, more fine that in
A. proteus; mitochondrial heteromorphism is known;
honeycomb-like nuclear lamina (Kalinina et al., 1987).
This species is generally smaller and less wrinkled
than A. proteus. Identification require special
attention. As far as I know, the only record of this
species is from the original isolation site.TEM of the cell
coat is obligate for correct identification; nuclear DNA
content of this species is about half that of A. proteus
(Kalinina et al., 1987). Incompatible with other species
of the genus Amoeba by nuclear transplantation.
Freshwater, North-Western Russia.
Cultivation: PJ medium + Tetrahymena. Can be
maintained for some time in PJ + wheat (rise) grain, in
presence of Tetrahymena.
The type strain is available from
Collection of Algae and Protozoa, CEH Windermere, UK.
Basic references for identification:
Note that the keys by Page from 1988 and 1991 harbour slightly
different sets of illustrations for this species.
- Kalinina L.V., Afon'kin S.Yu., Gromov D.B.,
Khrebtukova I.A. and Page F.C. 1987. Amoeba borokensis
n.sp., a rapidly dividing organism especially suitable
for experimental purposes. Arch. Protistenk.
- Page F.C. 1988. A new key to freshwater and soil
gymnamoebae. FBA, Ambleside. 122p.
- Page F.C. 1991. Nackte Rhizopoda. in: Protozoenfauna,
b.2. Gustav Fisher, Stuttgart. 3-172.
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