Amoeba leningradensis CCAP 1503/6.
Inverted NikonDiaphot, phase contrast.
Locomotive forms, nucleus and uroidal structures.
Amoeba leningradensis Page & Kalinina, 1984
Lentgh in locomotion 160-550 mkm (average about 360 mkm).
In locomotion amoebae are polypodial (polytactic) under favourable
conditions, but always with pronounced leading pseudopodium
or monopodial, nearly without longitudial wrinkles. Crystals
are truncate bipyramids, numerous, always giving amoebae
dark appearance. Floating form has several thick pseudopodia
of unequal length, radiating from the central mass of the cytoplasm.
Nucleus of granular type, spherical, slightly compressed
(thus appearing ovoidal), 21-29 mkm in diameter (average 25 mkm).
Surface coat consists of amorphous glycocalyx, less then 20
nm thick; mitochondrial heteromorphism not known, all mitochondria
have dark matrix; honeycomb-like nuclear lamina (Page & kalinina,
Amoebae of this species form less pseudopodia
than A. proteus and are much more smooth.
The cytoplasm always appears to be very dark, especially under
the dissectional microscope. Identification require special
attention. As far as I know, the only record of this
species is from the original isolation site.TEM of the cell
coat is obligate for correct identification.
Incompatible with other species
of the genus Amoeba by nuclear transplantation.
Note that the name "Metamoeba leningradensis" suggested
by Friz (1992: Arch. Protistenk. 142: 29-40) for this species
basing on the protein polymorphism is introduced
without any proper revision of the genus Amoeba and
without any proper diagnosis of the "new genus" Metamoeba.
Thus, the genus Metamoeba is invalid, and the combination
"Metamoeba leningradensis" should not be used!
Freshwater, North-Western Russia.
Cultivation: PJ medium + Tetrahymena. Can be maintained
for some time in PJ + wheat (rise) grain, in presence of
The type strain is available from
Collection of Algae and Protozoa, CEH Windermere, UK.
Basic references for identification:
Note that the keys by Page from 1988 and 1991 harbour slightly
different sets of illustrations for this species.
- Page F.C. and Kalinina L.V. 1984. Amoeba leningradensis
n. sp. (Amoebidae): a taxonomic study incorporating
morphological and physiological aspects.
Arch. Protistenk. 128: 37-53.
- Page F.C. 1988. A new key to freshwater and soil
gymnamoebae. FBA, Ambleside. 122p.
- Page F.C. 1991. Nackte Rhizopoda. in: Protozoenfauna,
b.2. Gustav Fisher, Stuttgart. 3-172.
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