
Chaos glabrum (original isolate).
UB1, DIC/Bright field.
Locomotive forms, nucleus and uroidal structures.
Photo: A.Smirnov

Chaos glabrum (original isolate).
1: nucleus, 2: two types of mitochondria; 3: nuclear lamina;
4: amorphous cell coat
TEM: A.Smirnov
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Chaos glabrum Smirnov & Goodkov, 1997 (original isolate)
Lentgh in locomotion: when polytactic - about 500 mkm, orthotactic
locomotive form 420-980 mkm (average 816 mkm).
Majority of amoebae orthotactic in locomotion (monopodial with distinct
lateral wrinkles), few are polytactic (polypodial).
Crystals are truncate bipyramids, spindles and cubes.
Floating form of radial type; several thick pseudopodia of unequal
lenght radiate from irregular central mass of the granuloplasm.
6-30 discoid, biconvex nuclei of granular type,
about 20 mkm in diameter.
Surface coat amorphous, 50-60 nm in thickness, consists of
two distinct layers; mitochondrial heteromorphism is known;
honeycomb-like nuclear lamina (Smirnov & Goodkov, 1997).
This species resembles Chaos nobile very much. The
differences are the floating form (Chaos nobile has a
very specific floating form (see Page, 1988)) and ultrastructure
of the cell coat. TEM of the cell coat is obligate for
identification, keep in mind that filaments in proteus-like
amoebae may coagulate under the EM fixation.
There is now available cultures of this species. All data
were obtained from the original isolate. Type slides (iron
haemotoxylin) are
deposited with the museum of preparations of the laboratory of
Invertebrate Zoology, Biological Research Inst. of St.Petersburg
State University, under the numbers #1994:704, #1994:705-706.
Known only from the original habitat.
Freshwater, North-Western Russia, Valamo island.
Cultivation: was maintained for some time in PJ +
wheat (rise) grain, in presence of small ciliates and
flagellates.
Basic references for identification:
- Smirnov A.V. and Goodkov A.V. 1997. Description of a large,
multinucleate lobose amoeba Chaos glabrum n.sp.
(Lobosea, Amoebidae), with notes on the diagnosis of the genus
Chaos. Acta Protozool. 36: 227-233.
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