Polychaos fasciculatum CCAP 1564/1
Locomotive forms. DIC.
Photo: A.Smirnov
Polychaos fasciculatum CCAP 1564/1
Nucleus, contractile vacuole and uroidal structures.
Photo: A.Smirnov
Polychaos fasciculatum CCAP 1564/1.
1: nucleus (tangential section through the piece of
nucleolar material makes an impression of
large nucleolus, 2: cell coat (filaments coagulated
under fixation, 3: nuclear lamina; 4: mitochondria
TEM: A.Smirnov
- Polypodial, "plamate" amoeba with short, parallel
pseudopodia and large fasciculate uroid (especially at the
beginning of locomotion). Lentgh in locomotion 65-140 mkm
(average 110 mkm), monopodial, clavate locomotive forms
may appear under low oxygene tension (Page, 1988).
Crystals are truncate bipyramids.
Floating form of radial type; several thick pseudopodia of unequal
lenght radiate from irregular central mass of the granuloplasm.
- Single spherical nucleus, 11-23 mkm in diameter, with
nucleolar material in few narrow peripheral lobes, sometimes -
in a single curved band, twisting inside the nucleus.
- Surface coat consists of very fine filaments, often
collupsing under unappropriate fixation.
- This species may be confused with Polychaos annulatum,
however the latter has very distinct nuclear structure. Careful
examination of 3D organisation of the nucleolus always excludes any
doubts about the species identification. Comparison of Polychaos
fasciculatum-like" amoebae was done by Smirnov & Goodkov (1998).
Culture Collection of Algae and Protozoa, CEH Windermere, UK). It is a "rise" culture with relatively low density of amoebae. - Freshwater, Europe. Cultivation: Rise + PJ medium and accompanying small protists. Can be maintained for some time in PJ + Tetrahymena.
- Freshwater, Europe. Cultivation: Rise + PJ medium and accompanying small protists. Can be maintained for some time in PJ + Tetrahymena.
- Smirnov A.V. and GoodkovA.V. 1998. Study of Polychaos annulatum Penard, 1902 comb. nov. (Gymnamoebia, Amoebidae) with taxonomical analysis of Polychaos fasciculatum-like species. Europ. J. Protistol. 34: 1-9.
- Page F.C. 1986. The genera and possible relationships of the family Amoebidae, with special attention to comparative ultrastructure. Protistologica. 22: 301-316.
- Page F.C. 1988. A new key to freshwater and soil gymnamoebae. FBA, Ambleside. 122p.
- Page F.C. 1991. Nackte Rhizopoda. in: Protozoenfauna, b.2. Gustav Fisher, Stuttgart. 3-172.